August 25th

2.00-3.00 pm Registration

3.00-3.15 – Welcome

3.15-4.00 – Opening lecture: Dan S. Tawfik, Weizmann Institute of Science, Israel – “The coevolution of proteins and their cofactors – some ancient finger prints and missing links”


Session I: Biotin, Thiamine, Pterin and SAM

Chair: Tadhg Begley and Dan S. Tawfik

4.00-4.40 – Keynote lecture: Tadhg Begley, Texas A&M University College Station, U.S.A. – “Radical SAM enzymes in cofactor biosynthesis”

4.40-5.10Yilin Hu,  University of California, U.S.A. – “Tracing the interstitial carbide of the nitrogenase cofactor"

5.10-5.40 Aurora Martinez, University of Bergen, Norway – “Pharmacological modulation of the tetrahydrobiopterin-dependent aromatic amino acid hydroxylases (AAAHs)”

5.40-5.50Paola Laurino, Weizmann  Institute of  Science, Israel – “Methyltransferase: a case study for cofactor engineering”

5.50-6.00Louis Luk, Cardiff School of Chemistry, U.K. – “Locating dynamic effects by studying enzyme isotopic hybrids”

6.30-8.00  – Get-together buffet


August 26th

8.30-9.00 am Registration

Session II: Pyridoxal phosphate (First part)

Chair: Gunter Schneider and Michael Dunn

9.00-9.40 – Keynote lecture: Robert Phillips, University of Georgia, U.S.A.  – “PLP chemistry and diversity”

9.40-10.10Barbara Cellini, University of Verona, Italy – “Pathogenic mutations of alanine-glyoxylate aminotransferase”  

10.10-10.20Klaus Schnackerz, University of Wuerzburg, Germany – “Crystal structure of D-serine dehydratase from Escherichia coli

10.20-10.30Tomokazu Ito, Nagoya University, Japan – “Catalytic mechanism of PLP- and Zn2+-dependent  D-serine dehydratase”

10.30-11.00 Coffee break


Session III: Flavins

Chair:  Yasuhisa Asano and Marco W. Fraaije

11.00-11.40 – Keynote lecture: Sandro Ghisla, University of  Konstanz, Germany – “Versatility of flavins and flavin enzymes and its molecular origin”

11.40-12.10Nigel S. Scrutton, University of  Manchester,  U.K. - “Coordinating redox chemistry through domain dynamics in flavoproteins”

12.10-12.20Helen Bell, University of Edinburgh, U.K. - “Kynurenine 3-monooxygenase – Domain mobility and ligand binding”

12.20-12.30Kenji Inagaki, Okayama University, Japan – “Engineering of substrate specificity of L-glutamate oxidase from Streptomyces sp.: directed mutagenesis of Arg305 residue”

12.30-12.40Paolo Sobrado, Virginia Tech, U.S.A. – “Chemical mechanism of flavin-dependent ornithine N5-hydroxylases”

12.40-3.00 pm Lunch and poster session


Session IV: Fe/S (First part)

Chair: Annalisa Pastore and Franco Bonomi

3.00-3.40 – Keynote lecture: Dennis Dean, Virginia Tech, U.S.A. – “Mechanistic features of nitrogenase - A complex metalloenzyme”

3.40-4.10Sandra Macedo Ribeiro, University of  Porto, Portugal – “IscR: The two faces of a [Fe-S] cluster biogenesis regulator”

4.10-4.20Takashi Tamura, Okayama University, Japan – “Molecular evolution of gas cavity in [NiFeSe]-hydrogenases resurrected in silico by ancestral sequence estimation and 3D modeling by molecular dynamics simulation”

4.20-4.50 Coffee break

Session V: Heme

Chair: Arwen Pearson and Stefano Bettati

4.50-5.30 – Keynote lecture: Thomas Barends, Radboud University Nijmegen, The Netherlands - “Unusual cofactors in ANAMMOX enzymes”

5.30-6.00Arwen Pearson, University of Hamburg, Hamburg, Germany – “Radiation damage as a tool to probe enzyme mechanism”

6.00-6.10Hisaaki Mihara, Ritsumeikan University, Japan – “The unique multiheme selenoprotein of the metal-reducing anaerobic bacterium Geobacter sulfurreducens

6.10-6.20Alexey Topunov, Bach Institute of Biochemistry, Russia – “Extremely high peroxidase activity of plant hemoglobin”

6.30Guided tour of Parma


August 27th

Session VI: Biotechnological, biomedical and pharmaceutical applications of cofactors and cofactors-enzymes

Chair: Tatyana Demidkina and Paul Cook

9.00-9.30Maria Laura Bolognesi, University of Bologna, Italy – “Targeting GAPDH with small molecule inhibitors: 2-phenoxynaphthoquinones as lead candidates for Trypanosomatid diseases”

9.30-10.00Tatyana Demidkina, Russian Academy of Sciences, Russia – ”Methionine gamma-lyase as a target in pathogens and for cancer therapy”

10.00-10.20Yasuhisa Asano, Toyama Prefectural University, Japan  – “Creation of R-stereoselective amine oxidase from porcine kidney D-amino acid oxidase and its use in deracemization of alpha-methylbenzylamine”

10.20-10.30Elena Rosini, University of Insubria, Varese, Italy – “A new biotechnological application of glycine oxidase: evolution of novel enzyme-based biosensors for detection of achiral amino acids”

10.30-11.00 – Coffee break


Session VII: Pyridoxal phosphate (Second part)

Chair: Paola Dominici and Roberto Contestabile

11.00-11.30 – Gunter Schneider, Karolinska Institute, Sweden – “Enzymes of mycobacterial cysteine biosynthesis as potential drug targets”

11.30-12.00Barbara Campanini, University of Parma, Italy – “Moonlighting CysK: from cysteine synthesis to toxin activation, the many faces of a potential antibiotic target”

12.00-12.10 –  Takashi Takenaka, Nagoya University, Japan – “Characterization of a novel PLP-binding transcriptional regulator of Brevibacillus brevis

12.10-12.20Paola Dominici, University of Verona, Italy – “Functional roles of the hexamer organization of plant glutamate decarboxylase”

12.20-12.30Orna Almog, Ben-Gurion University of the Negev, Israel – “Structure - function relationship in E. coli tryptophanase: The role of residues 15, 59 and 298 on its cold lability”

12.30-3.00 pm Lunch and poster session


Session VIII: Flavin and pyridoxal phosphate cofactors and pathologies

Chair: Loredano Pollegioni and Nigel S. Scrutton

3.00-3.30Herman Wolosker,  Technion Israel Institute of Technology, Israel – “Regulation of serine racemase by the serine shuttle: A new type of glia-neuron crosstalk affecting neurotransmission and neurodegeneration”

3.30-4.00 – JeanPierre Mothet, Aix Marseille University, CNRS, France – “Deciphering the role of D-amino acid oxydase to NMDA receptor synaptic functions”

4.00-4.30Silvia Sacchi, University of Insubria, Italy – “Modulation of D-amino acid oxidase activity and schizophrenia susceptibility”

4.30-4.40Stefano Bruno, University of Parma, Italy –  “Fine tuning of serine racemase activity”

4.40-4.50Nobuyoshi Esaki,  Kyoto University, Japan – “Physiological role and reaction mechanism of selenocysteine lyase: Unique vitamin B6 enzyme acting specifically on selenocysteine”

4.50-5.00Alessio Fiascarelli, Sapienza University of Rome, Italy – “Human serine hydroxymethyltransferase as chemotherapeutic target: inhibition by 3-bromopyruvate, a promising antitumoral compound”

5.00-5.30Coffee break


Session IX: Quinones

Chair: Carrie Wilmot and Nobuyoshi Esaki

5.30-6.00 – Keynote lecture: Carrie Wilmot, University of Minnesota, U.S.A. – “Posttranslational biosynthesis of the protein-derived cofactor tryptophan tryptophylquinone"

6.00-6.30Antonio Sanchez-Amat, University of  Murcia, Spain – “L-lysine-epsilon oxidase from Marinomonas mediterranea, the first amino acid oxidase containing a quinone cofactor”

6.30-6.40Seiji Okazaki, Toyama Prefectural University, Japan –“ A novel structure of a cysteine tryptophylquinone-dependent oxidase, L-lysine ε-oxidase from Marinomonas mediterranea

6.40-6.50Tadashi Nakai, Osaka University, Japan – “Complete journey of quinohemoprotein amine dehydrogenase from genes to periplasm”

6.50-7.00Takeshi Murakawa, Osaka Medical College, Japan – “Conformational flexibility of the topa quinone cofactor in copper amine oxidase revealed by site-specific mutagenesis of the neighboring conserved asparagine residue”

8.00-11.00 pm – Social dinner


August 28th

Session X: Fe/S (Second part)

Chair: Sandra Macedo Ribeiro and Dennis Dean

9.00-9.30Annalisa Pastore, King’s College London, U.K. – “The role of the scaffold protein IscU retains in Fe-S cluster assembly”

9.30-10.00Silke Leimkühler, University of  Potsdam, Germany – “Connecting the biosynthesis of the molybdenum cofactor, Fe-S clusters and tRNA thiolation in humans”

10.00-10.30 – Juan Fontecilla-Camps, CEA-CNRS, Grenoble, France – “X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters”

10.30-11.00 – Coffee break


Session XI:  Pyridoxal phosphate (Third part)

Chair:  Herman Wolosker and Robert Phillips

11.00-11.30Leonard Mueller, University of  California,  California,  U.S.A. - “Protonation states of catalytic intermediates at the beta-subunit of tryptophan synthase from solid-state NMR spectroscopy"

11.30-12.00Roberto Contestabile, University of Rome “La Sapienza”, Italy – "PdxR: a pyridoxal 5’-phosphate-dependent transcriptional regulator involved in the control of PLP biosynthesis“

12.00-12.15Ivo Tews, University of Southampton, U.K. – “Study of vitamin B6 biosynthesis by PLP synthase”

12.15-12.30Michael Dunn, University of  California, U.S.A. -  “Conformational switching and catalysis in the structure and function of tryptophan synthase”

12.30-2.30 pmLunch and poster session


Session XII: NAD
Chair: Nadia Raffaelli and Sandro Ghisla

2.30-3.00 – Keynote lecture: Andreas Guse, University Medical Centre Hamburg-Eppendorf, Germany – “Second messengers derived from NAD: metabolism, mechanism of calcium release and roles in T-lymphocytes and cardiac myocytes"

3.00-3.30Nadia Raffaelli, Polytechnic University of Marche, Italy  – “Dissecting NAD biogenesis in mammalian cells”

3.30-4.00Laura Conforti, University of Nottingham, U.K. – “NAD biosynthetic pathway: road to neuroprotection”

4.00-4.10  – Alexandre Marbaix, Université Catholique de Louvain, Belgium – ”An extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair”

4.10-4.20Luca Sorrentino, University of Milan, Italy –“ The G308E variant of the apoptosis inducing factor, responsible of a rare encephalopathy, is hampered in NAD+/H binding”

4.20-4.50Coffee break


Session XIII: Metals

Chair: Paola Turano and John Dawson
4.50-5.20 – Keynote lecture: John Dawson, University of South Carolina, U.S.A. – “Dioxygen binding and the switch to peroxide activation by the globin sea worm peroxidase, Amphitrite ornata dehaloperoxidase”

5.20-5.50Paola Turano, University of Florence, Italy – “Iron trafficking in the ferritin nanocage”

5.50-6.00Toshihide Okajima, Osaka University, Japan – “Sub-atomic resolution structure of a bacterial copper amine oxidase reveals a putative dioxygen entry route to the active site”

6.00-6.30 – Closing talk: Marco W. Fraaije, University of  Groningen, The Netherlands – “Creating new flavoenzymes by flavin cofactor engineering”

6.30-6.40 – Final remarks